Iinfluence of the Immunosuppressant Tacrolimus (fk-506) on the Flexural Strength of Femur: a Study in Rats
نویسندگان
چکیده
OBJECTIVE To evaluate the resistance to femoral fractures among rats treated with the immunosuppressant tacrolimus FK-506 and compare these to untreated rats and rats treated with placebo. METHODS Ninety male Wistar rats were used. The animals were nine weeks old and weighed between 220 g and 280 g. The immunosuppressive agent tacrolimus was used in this study at a dose of 2 mg/kg/day, administered orally. The suspension was administered using an insulin syringe, and the maintenance therapeutic dose was sufficient to maintain the immunosuppressive activity. The animals were randomly divided into three groups (n = 30): group 1, no substance administered; group 2, administration of the immunosuppressant tacrolimus FK-506; and group 3, administration of the vehicle alone. Treatment with FK-506 was administered for 28 days. Total leukocyte counts and differential counts (lymphocytes, monocytes, eosinophils and neutrophils) were evaluated in order to monitor the immunosuppressive effect. Bone densitometry analysis by means of dual-energy x-ray absorptiometry (DXA) was also performed before and after administration of the drug. To evaluate the resistance to flexion, a support device was developed so that mechanical tests using an EMIC universal testing machine could be carried out. RESULTS The results from the flexion resistance tests showed statistical differences between groups 1 and 2 (p = 0.001) and between groups 2 and 3 (p = 0.001). No statistical difference was found between groups 1 and 3 (p = 0.995). CONCLUSIONS The femurs of rats treated with the immunosuppressive agent had lower mechanical strength than did those of normal rats and those that received placebo.
منابع مشابه
Intra-CA1 administration of FK-506 (tacrolimus) in rat impairs learning and memory in an inhibitory avoidance paradigm
Objective(s): Calcineurin (CN) is a main phosphatase and a critical regulator of cellular pathways for learning, memory, and plasticity. The FK-506 (tacrolimus),a phosphatase inhibitor, is a fungal-derived agent and a common immune suppressant extensively used for tissue transplantation. To further clarify the role of CN in different stages oflearning and memory the main aim of this study was t...
متن کاملSirolimus and tacrolimus rather than cyclosporine A cause bone loss in healthy adult male rats
The aim of this work was to study the effects of cyclosporine (CsA), tacrolimus (FK-506), and rapamycin (RAPA) on bone mass, femoral microstructure, femoral biomechanical properties, and bone remodeling in healthy adult male rats. Forty-eight 5-month-old male Wistar rats were used. CsA (2 mg/kg/day), FK-506 (3 mg/kg/day), RAPA (1.25 mg/kg/day), or water (0.5 ml/rat/day, control group) were admi...
متن کامل[The effect of FK 506, an immunosuppressant, on cerebral infarction volume in focal cerebral ischemia in rats].
The effect of FK 506, an immunosuppressant which is widely used in the transplantation of liver, kidney and bone marrow, on cerebral infarction volume in transient focal ischemia was investigated. Focal cerebral ischemia was produced by 2 h occlusion of the left middle cerebral artery in rats. FK 506 (0.3 mg/kg body weight) and vehicle were administered intravenously immediately after induction...
متن کاملFK 506--an effective immunosuppressant in achieving long-term functional islet allograft survival in diabetic rats.
TRANSPLANTED pancreatic islet tissue has been shown to restore normoglycemia and prevent the development of chronic complications in diabetic animals. 1 The application of alloand xenotransplantation of pancreatic islets for the treatment of diabetes is hindered by immune rejection. FK 506, a new immunosuppressive agent, has been demonstrated to be effective in the prolongation of survival of h...
متن کاملEffect of FK 506 on glucose metabolism and insulin secretion in normal rats.
FK 506 is a powerful immunosuppressant currently being investigated in clinical transplantation. I In vivo administration of this drug resulted in the prolongation of skin, liver, kidney, and heart allografts in large and small animal models.2•3 The immunosuppressive effect has also been shown recently to be effective in the prevention of development of diabetes in BB rats.4 In baboons. treatme...
متن کامل